Journal: PLoS ONE

Article Title: LncRNA HOTAIR promotes proliferation, invasion and migration in NSCLC cells via the CCL22 signaling pathway

doi: 10.1371/journal.pone.0263997

Figure Lengend Snippet: A, B: the expression of HOTAIR and CCL22 mRNA were detected by RT-qPCR. C: CCL22 protein analyzed by ELISA assay. ****, P<0.001.

Article Snippet: The concentration of CCL22 in the supernatant was tested according to the instructions of ELISA kit operation (CUSABIO, CSB-E04660h).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: LncRNA HOTAIR promotes proliferation, invasion and migration in NSCLC cells via the CCL22 signaling pathway

doi: 10.1371/journal.pone.0263997

Figure Lengend Snippet: A: The HOTAIR and CCL22 mRNA expression examined by RT-qPCR B: Cell apoptosis detected by Flow cytometry. C: The cell proliferation tested by MTS assay. D: The CCL22 protein expression tested by ELISA. E: The cell migration and invasion detected by Transwell assay. **, P<0.05; ***, P<0.01; ****, P<0.001;ns, no significance.

Article Snippet: The concentration of CCL22 in the supernatant was tested according to the instructions of ELISA kit operation (CUSABIO, CSB-E04660h).

Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, MTS Assay, Enzyme-linked Immunosorbent Assay, Migration, Transwell Assay

Journal: Cytokine

Article Title: An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium

doi: 10.1016/j.cyto.2017.05.023

Figure Lengend Snippet: Most abundant novel chemokines present in VWF positive vessels in RA compared to non-RA synovia.

Article Snippet: ELISA was performed using CCL7, CCL14, CCL16 and CCL22 DuoSet ELISA kits (R&D Systems, UK); wash buffer, reagent diluent, substrate solution and stop solution (all R&D Systems) were used throughout according to the manufacturer’s instructions.

Techniques:

Journal: Cytokine

Article Title: An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium

doi: 10.1016/j.cyto.2017.05.023

Figure Lengend Snippet: CCL16 and CCL22 in RA and non-RA synovium. For RA CCL16 (A, B, C), CCL22 (G, H, I) and non-RA CCL16 (D, E, F), CCL22 (J, K, L) chemokines are shown in red with von-Willebrand factor (VWF) in green and DAPI in blue, with merged images to illustrate colocalisation. Scale bars in A-C and G-I are 200 µm, and in D-F and J-L 50 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: ELISA was performed using CCL7, CCL14, CCL16 and CCL22 DuoSet ELISA kits (R&D Systems, UK); wash buffer, reagent diluent, substrate solution and stop solution (all R&D Systems) were used throughout according to the manufacturer’s instructions.

Techniques:

Journal: Cytokine

Article Title: An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium

doi: 10.1016/j.cyto.2017.05.023

Figure Lengend Snippet: CCL7, CCL14, CCL16 and CCL22 comparisons for RA and OA SF and serum. (A) shows CCL7 levels to be higher in RA SF compared to OA SF, RA serum and OA serum at p < 0.01. (B) shows the presence of CCL14 in RA and OA. (C) shows CCL16 to be higher in RA serum compared to RA SF and OA SF at p < 0.001.(D) shows CCL22 as increased in RA serum compared to OA SF at p < 0.05. The vertical bar indicates overall range, the box shows interquartile range with horizontal line in the box showing median (RA n = 17, OA n = 7) Kruskal- Wallis ANOVA was used followed by the Dunn’s multiple comparison test * p = <0.05, ** p = <0.01, *** p = <0.001.

Article Snippet: ELISA was performed using CCL7, CCL14, CCL16 and CCL22 DuoSet ELISA kits (R&D Systems, UK); wash buffer, reagent diluent, substrate solution and stop solution (all R&D Systems) were used throughout according to the manufacturer’s instructions.

Techniques: Comparison