Journal: Journal of Inflammation Research

Article Title: Alpha-Momorcharin Inhibits Proinflammatory Cytokine Expression by M1 Macrophages but Not Anti-Inflammatory Cytokine Expression by M2 Macrophages

doi: 10.2147/JIR.S372306

Figure Lengend Snippet: Inflammatory cytokines and anti-inflammatory cytokines expression in M0, M1 and M2 macrophages. ( A ) TNF- α; ( B ) IL-1β; ( C ) IL-6; ( D ) IL-8; ( E ) MCP-1; ( F ) MIP-1α; ( G ) IL-RA; ( H ) IL-10; ( I ) TGF-β1; ( J ) EGF; ( K ) VEGF-A; ( L ) CCL22. The Orange-red columns are the M0-type macrophage groups, which was not treated with LPS; the blue columns are the M1-type macrophage groups; the purple columns are the M2-type macrophage groups. These macrophages were treated with 0.3 μg/mL α-MMC for 0 hours, 2 hours, 8 hours, 24 hours, or 48 hours. The data shown are individual values with the mean ± SEM; n = 5. * P < 0.05 significantly different from the M0 macrophage group of 0 hours; # P < 0.05 significantly different from the M1 macrophage group of 0 hours; n.s. No significant difference from the control group. One-way analysis of variance, Tukey’s multiple comparison tests.

Article Snippet: The following ELISA kits produced by Immunoway Co., Ltd. were used: Human TNF-α ELISA Kit (KE1372), Human IL-1β ELISA Kit (KE1379), Human IL-6 ELISA Kit (KE1368), Human IL-8 ELISA Kit (KE1360), Human MIP-1α ELISA Kit (KE 1052), Human MCP-1 ELISA Kit (KE1511), Human IL-1RA ELISA Kit (KE1030), Human IL-10 ELISA Kit (KE1369), Human EGF ELISA Kit (KE1012), Human VEGF-A ELISA Kit (KE1374), Human TGF-β ELISA Kit (KE1373), and Human CCL22 ELISA Kit (KE1203).

Techniques: Expressing